Journal: iScience

Article Title: Loss of Ezh2 promotes M2-like macrophage polarization in hepatocellular carcinoma

doi: 10.1016/j.isci.2026.115180

Figure Lengend Snippet: ChIP-qPCR analysis of H3K27me3 enrichment on promoter regions of Pcx , Pkm , and Spi1 in Hepa1-6 CM-treated WT or Ezh2 KO BMDMs ChIP was performed using an anti-H3K27me3 antibody or normal rabbit IgG control. DNA fragments were subjected to qPCR using primer sets covering the promoter regions of each gene. Data are expressed as relative enrichment normalized to the input chromatin. Data represented as mean ± S.E.M. of three biological replicates. ∗ p < 0.05 Statistical test was conducted by two-way ANOVA.

Article Snippet: Rabbit anti-mouse H3K27me3 antibody , Cell Signaling Technology , 9733.

Techniques: ChIP-qPCR, Control

Journal: iScience

Article Title: Loss of Ezh2 promotes M2-like macrophage polarization in hepatocellular carcinoma

doi: 10.1016/j.isci.2026.115180

Figure Lengend Snippet: ChIP-qPCR analysis of H3K27me3 enrichment on promoter regions of Pcx , Pkm , and Spi1 in Hepa1-6 CM-treated WT or Ezh2 KO BMDMs ChIP was performed using an anti-H3K27me3 antibody or normal rabbit IgG control. DNA fragments were subjected to qPCR using primer sets covering the promoter regions of each gene. Data are expressed as relative enrichment normalized to the input chromatin. Data represented as mean ± S.E.M. of three biological replicates. ∗ p < 0.05 Statistical test was conducted by two-way ANOVA.

Article Snippet: Briefly, BMDMs were crosslinked with 1% formaldehyde to stabilize protein-DNA complexes, and chromatin fragments were immunoprecipitated using a rabbit anti-H3K27me3 antibody (Cat. 9733, Cell Signaling Technology) or the kit-provided normal rabbit IgG antibody (Cat. 2729, Cell Signaling Technology) for ChIP-qPCR analysis.

Techniques: ChIP-qPCR, Control